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991.
Sex‐biased gene expression during the adult stage of the Indian meal moth Plodia interpunctella 下载免费PDF全文
Sexual dimorphisms are primary regulated by sex‐biased gene expression. In the present study, using real‐time polymerase chain reaction, we determined the expression profiles of nine genes associated with development, metabolism, stress, and defense throughout adulthood of the Indian meal moth Plodia interpunctella, a global pest of stored food products. Most genes were differentially expressed in a sex‐biased manner during the adult lifespan of the moth. Expression of the heat shock protein genes hsp25 and hsp90 and the antioxidant gene thioredoxin peroxidase (Tpx) was highly female biased, whereas the expression of a gene related to host development (ecdysone receptor [EcR]) and two genes associated with immunity (β‐glycan recognition protein [βgrp] and prophenoloxidase [ProPO]) was male biased. In contrast, the expression of hsp70, glucose‐regulated protein 78 (grp78) and ultraspiracle (USP) was not sex biased. The results of the present study provide important insights into the role of sex‐biased genes in the physiology and behavior of P. interpunctella. 相似文献
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Vanessa J. Shamrock Jérôme F.L. Duval George G. Lindsey & Fabien Gaboriaud 《FEMS yeast research》2009,9(3):391-399
In this study, we investigate the electrohydrodynamic and nanomechanical characteristics of two Saccharomyces cerevisiae yeast strains, a wild-type (WT) strain and a strain overexpressing (OE) Hsp12p, in the presence and absence of hydrophobic Congo red compound. By combining these two advanced biophysical methods, we demonstrate that Hsp12p proteins are mostly located within a thin layer ( c . 10 nm thick) positioned at the external side of the cell wall. However, this Hsp12p-enriched layer does not prevent Congo red from entering the cell wall and from interacting with the chitin therein. The entrance of Congo red within the cell wall is reflected in an increase of the turgor pressure for the OE strain and a decrease of that for the WT strain. It is shown that these opposite trends are consistent with significant modulations of the water content within the cell wall from/to the cytoplasm. These are the result of changes in the hydrophobicity/hydrophilicity balance, as governed by the intertwined local concentration variations of Congo red and Hsp12p across the cell wall. In particular, the decrease of the turgor pressure in the case of WT strain upon addition of Congo red is shown to be consistent with an upregulation of Hsp12p in the close vicinity of the plasma membrane. 相似文献
997.
Three extremely diverse groups of unicellular eukaryotes comprise the Alveolata: ciliates, dinoflagellates, and apicomplexans. The vast phenotypic distances between the three groups along with the enigmatic distribution of plastids and the economic and medical importance of several representative species (e.g. Plasmodium, Toxoplasma, Perkinsus, and Pfiesteria) have stimulated a great deal of speculation on the early evolutionary history of alveolates. A robust phylogenetic framework for alveolate diversity will provide the context necessary for understanding the basic biological properties of the group and for developing appropriate strategies for management. We addressed the earliest stages of alveolate evolution by sequencing heat shock protein 90 (hsp90) genes from several ciliates, apicomplexans, and dinoflagellates, including key species thought to represent early diverging lineages: Oxyrrhis marina, Perkinsus marinus, Cryptosporidium parvum, and the eugregarine Monocystis agilis. Moreover, by sequencing the actin gene from Monocystis, we were able to examine the sister relationship between gregarines and cryptosporidians with a three‐protein concatenated data set (hsp90, actin, and β‐tubulin). Phylogenetic analyses of the hsp90 data set provided a robust topology for alveolate relationships: Alveolates were monophyletic and apicomplexans and dinoflagellates formed sister groups to the exclusion of ciliates. Oxyrrhis formed the earliest diverging sister lineage to the “core” dinoflagellates, and Perkinsus formed the earliest diverging sister lineage to the Oxyrrhis–dinoflagellate clade. This topology was strongly supported inall analyses and by a unique indel shared by Oxyrrhis and dinoflagellates. A sister relationship between Cryptosporidium and Monocystis was weakly supported by the hsp90 data set but strongly supported by the three‐protein concatenated data set. 相似文献
998.
The kinetoplastids Leishmania major, Trypanosoma brucei and Trypanosoma cruzi are causative agents of a diverse spectrum of human diseases: leishmaniasis, sleeping sickness and Chagas' disease, respectively. These protozoa possess digenetic life cycles that involve development in mammalian and insect hosts. It is generally accepted that temperature is a triggering factor of the developmental programme allowing the adaptation of the parasite to the mammalian conditions. The heat shock response is a general homeostatic mechanism that protects cells from the deleterious effects of environmental stresses, such as heat. This response is universal and includes the synthesis of the heat-shock proteins (HSPs). In this review, we summarize the salient features of the different HSP families and describe their main cellular functions. In parallel, we analyse the composition of these families in kinetoplastids according to literature data and our understanding of genome sequence data. The genome sequences of these parasites have been recently completed. The HSP families described here are: HSP110, HSP104, group I chaperonins, HSP90, HSP70, HSP40 and small HSPs. All these families are widely represented in these parasites. In particular, kinetoplastids possess an unprecedented number of members of the HSP70, HSP60 and HSP40 families, suggesting key roles for these HSPs in their biology. 相似文献
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Fiocco D Capozzi V Goffin P Hols P Spano G 《Applied microbiology and biotechnology》2007,77(4):909-915
The effect of overproducing each of the three small heat shock proteins (Hsp; Hsp 18.5, Hsp 18.55, and Hsp 19.3) was investigated
in Lactobacillus plantarum strain WCFS1. Overproduction of the three genes, hsp 18.5, hsp 18.55, and hsp 19.3, translationally fused to the start codon of the ldhL gene yielded a protein of approximately 19 kDa, as estimated from Tricine
sodium dodecyl sulfate–polyacrylamide gel electrophoresis in agreement with the predicted molecular weight of small Hsps.
Small Hsp overproduction alleviated the reduction in growth rate triggered by exposing exponentially growing cells to heat
shock (37 or 40°C) and cold shock (12°C). Moreover, overproduction of Hsp 18.55 and Hsp 19.3 led to an enhanced survival in
the presence of butanol (1% v/v) or ethanol (12% v/v) treatment suggesting a potential role of L. plantarum small Hsps in solvent tolerance. 相似文献